Lipid metabolism disorders of hybrid grouper (?Epinephelus fuscointestinestatus × ?E. lanceolatu) induced by high-lipid diet

This study investigated the effect of lipid levels on growth, body composition, metabolism enzymes activity and related genes expression in hybrid grouper (? Epinephelus fuscointestinestatus × ? E. lanceolatu ). Three diets with different 6% (low-lipid feed, LF), 11% (medium-lipid MF) 16% (high-lipid HF) were designed each diet was fed to triplicate groups 30 fish (6.73 ± 0.00g) for eight weeks. The results revealed following. 1) maximum weight gain rate (WGR), specific growth (SGR), protein efficiency ratio (PER) lowest feed conversion (FCR) values found HF group. There no significant difference survival (SR) among ( P > 0.05). 2) Condition factor (CF), hepatosomatic index (HSI) visceralsomatic (VSI) increased increasing dietary lipid. 3) Crude content whole-body muscle significantly as level < 4) Principal component analysis fatty acids showed that sum n-3 polyunsaturated (?n-3PUFA)/sum n-6 (?n-6PUFA) higher group than other two 5) Biochemical serum indexes triglycerides (TG), total cholesterol (TC), alkaline phosphatase (AKP) low-density lipoprotein-cholesterol (LDL-C) but lower high-density (HDL-C) 6) Liver biochemical indicators MF had lipoprotein lipase (LPL), hepatic (HL) triglyceride (ATGL) acid synthase (FAS) very-low density (VLDL-C) level. 7) relative lpl ), adipocyte-type acid-binding a-fabp liver-type l-fabp heart-type h-fabp ) hormone-sensitive hsl liver 0.05), while fas gene expressed at 8) Histological oil-red sections larger more droplets those diets. It concluded better performance this not conducive progress metabolism, resulting accumulation affecting quality fish. provides useful information formulation environmentally friendly cost-effective grouper.